Bacteriome from Pinus arizonica and P. durangensis: Diversity, Comparison of Assemblages, and Overlapping Degree with the Gut Bacterial Community of a Bark Beetle That Kills Pines
نویسندگان
چکیده
Symbioses between plants and microorganims have been fundamental in the evolution of both groups. The endophytic bacteria associated with conifers have been poorly studied in terms of diversity, ecology, and function. Coniferous trees of the genera Larix, Pseudotsugae, Picea and mainly Pinus, are hosts of many insects, including bark beetles and especially the Dendroctonus species. These insects colonize and kill these trees during their life cycle. Several bacteria detected in the gut and cuticle of these insects have been identified as endophytes in conifers. In this study, we characterized and compared the endophytic bacterial diversity in roots, phloem and bark of non-attacked saplings of Pinus arizonica and P. durangensis using 16S rRNA gene pyrosequencing. In addition, we evaluated the degree of taxonomic relatedness, and the association of metabolic function profiles of communities of endophytic bacteria and previously reported gut bacterial communities of D. rhizophagus; a specialized bark beetle that colonizes and kills saplings of these pine species. Our results showed that both pine species share a similar endophytic community. A total of seven bacterial phyla, 14 classes, 26 orders, 43 families, and 51 genera were identified. Enterobacteriaceae was the most abundant family across all samples, followed by Acetobacteraceae and Acidobacteriaceae, which agree with previous studies performed in other pines and conifers. Endophytic communities and that of the insect gut were significantly different, however, the taxonomic relatedness of certain bacterial genera of pines and insect assemblages suggested that some bacteria from pine tissues might be the same as those in the insect gut. Lastly, the metabolic profile using PICRUSt showed there to be a positive association between communities of both pines and insect gut. This study represents the baseline into the knowledge of the endophytic bacterial communities of two of the major hosts affected by D. rhizophagus.
منابع مشابه
Suitability of some southern and western pines as hosts for the pine shoot beetle, Tomicus piniperda (Coleoptera: Scolytidae).
The pine shoot beetle, Tomicus piniperda (L.), is an exotic pest that has become established in North America. Discovered in Ohio in 1992, it has since been found in at least 13 states and parts of Canada. The beetle can cause significant growth loss in pines, and it represents a potential threat to trees in areas where it has not yet become established. To evaluate this threat to native pines,...
متن کاملCOMPARISON OF EPICUTICULAR WAX ON NEEDLES AND STEMS OF Pinus eldarica WITH ITS TWO NATURALLY GENERATED FORMS
Plant cuticles are covered by epicuticular waxes with considerable ultrastructural and chemical diversity and have great systematic significance. Pinus elderica is a rare pine found naturally only in desert environment southeast of Tbilisi (Georgia). This tree have been probably introduced to Iran about 800 years ago and gradually altered in both shape and size in Nashtifan-Khaf, and changed in...
متن کاملLead and Cadmium Concentrations in Throughfall of Pinus eldarica
This research was carried out in order to quantify throughfall (TF) and interception loss (I) and to compare the chemical composition of TF, i.e. lead (Pb) and cadmium (Cd) as well as electrical conductivity (EC) and pH beneath Pinus eldarica and Cupressus arizonica plantations and the open field rainfall. The research was accomplished in the Chitgar Forest Park, a semi-arid polluted urban area...
متن کاملEvaluation of acute and sub-acute toxicity of Pinus eldarica bark extract in Wistar rats
Objective: Pinus eldarica (P. eldarica) is one of the most common pines in Iran which has various bioactive constituents and different uses in traditional medicine. Since there is no documented evidence for P. eldarica safety, the acute and sub-acute oral toxicities of hydroalcoholic extract of P. eldarica bark were investigated in male and female Wistar rats in this study. Materials and Method...
متن کاملMountain Pine Beetle Dynamics and Reproductive Success in Post-Fire Lodgepole and Ponderosa Pine Forests in Northeastern Utah
Fire injury can increase tree susceptibility to some bark beetles (Curculionidae, Scolytinae), but whether wildfires can trigger outbreaks of species such as mountain pine beetle (Dendroctonus ponderosae Hopkins) is not well understood. We monitored 1173 lodgepole (Pinus contorta var. latifolia Doug.) and 599 ponderosa (Pinus ponderosa Doug. ex Law) pines for three years post-wildfire in the Ui...
متن کامل